The targeting vector inserted into the locus a CAG promoter, floxed STOP sequence, Venus reporter gene, tripleF2A (three tandem repeats of foot-and-mouth disease 2A peptide sequence), Sdk2 gene, WPRE sequence and FRT-flanked neomycin resistance cassette. Flp-mediated recombination removed the neomycin resistance cassette. (J:225578)