一个敲除的诺卡因抗性基因座替换了exon 4。通过Cre介导的重组,去除了选择性基因座。 Western blot结果显示睾丸中没有检测到蛋白质表达。(来源:J:223053)
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A floxed neomycin resistance cassette replaced exon 4. Cre-mediated recombination removed the selection cassette. Western blot analysis confirmed the absence of protein expression in testes. (J:223053)