在exon 1和相邻上游的内含子序列中,替换了新霉素抗性基因 cassette, Western blot结果显示大脑中没有蛋白表达(来源:J:225714)
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A neomycin resistance cassette replaced a part of the ATG in exon 1 and adjacent upstream intronic sequence. Western blot analysis confirmed the absence of protein expression in the brain. (J:225714)