β-geo选择 cassette 替换了exon 2的部分和整个exon 3。胚胎提取物的 Western blot 分析确认了纯合突变胚胎中没有蛋白质表达。(来源:J:222380)
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A beta-geo selection cassette replaced part of exon 2 and all of exon 3. Western blot analysis of embryonic extracts confirmed the absence of protein expression in homozygous mutant embryos. (J:222380)