锌指内切酶被用来产生了敲除等位基因。在第428行中,第6外显子有8个碱基的缺失。 Western blot分析证实了没有蛋白质表达。(来源:J:225041)
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Zinc finger endonucleases were used to generate a knock-out allele. Lines 428 contains an 8 base pair deletion in exon 6. Western blot analysis confirmed the absence of protein expression. (J:225041)