来自项目Arap3-7000J-M3454的这个等位基因是在Jackson实验室通过注入Cas9 RNA和3个指导序列(AGGGGTCTGATCCCGAGGAG、GCATCAGTGCTACAGGACAC、AAAGTCTGAGGCTTGGACAG)产生的,这些序列导致了175bp的缺失,起始于18号染色体负链位置37,997,244bp的内含子2,终止于37,996,470bp的exon 3后,序列内容为TTTGGGCATTCTTCTTTGAAAGAGAT。这个变异导致了exon 2的删除,包括起始翻译的序列、中间的内含子以及exon 3中的11bp,预测为无功能的等位基因。(J:188991)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6NJ
Endonuclease-mediated
基因内删除
不确定
1
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Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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