Cacna1c^tm2.1Wac

An S1700A mutation followed by an FRT flanked neo cassette were inserted in exon 41 via homologous recombination. Flp mediated recombination removed the neo cassette. The Ala substitution for Ser-1700 does not substantially affect native channel expression or distribution, the stoichiometry of the distal (dCT) and proximal C terminus (pCT) in the autoinhibitory complex, or the overall beta-adrenergic signaling, as assessed by phosphorylation of S1928 in response to isoproterenol. (J:216746)