Wnt10b^{tm1(cre/ERT2)Amc}

An exchange vector was designed to be inserted downstream of exon 1 of the gene using dual-recombinase mediated cassette exchange (dRMCE). Specifically, the exchange vector contained (from 5 to 3) an FRT site, a viral F2A oligopeptide (to mediate ribosomal skipping), an enhanced green fluorescent protein sequence, a viral T2A oligopeptide, and a Cre-ERT2 sequence (Cre recombinase fused to a G525R mutant form of the mouse estrogen receptor ligand-binding domain). A rox-flanked puromycin resistance cassette and a loxP site were inserted at the 3' end of the exchange vector. (J:159099)