这个构建包含了鸡β-actin启动子(带有CMV即时早期增强子),驱动Cox8a(细胞色素c氧化酶亚基 VIIIa)的mitochondrial靶向序列,与pH敏感的荧光蛋白Keima连接。它被设计成attB位点簇周围,这些位点是通过微注射,连同PhiC31整合酶mRNA,注入来自Igs2tm3.1Luo小鼠的卵母细胞的核中。Igs2tm3.1Luo突变体有三个串联的attP位点。PhiC31整合酶能催化attB和attP位点之间的重组。(来源:J:101977)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Gene Expression
Related Disease
Reference
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Targeted
插入
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19

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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