在第8外显子通过同源重组,插入了一个Lac0-SA-IRES-lacZ-neo/kan cassette,替换了100bp的基因组序列。 Western blot结果显示P1纯合突变小鼠脑组织中没有检测到蛋白质。(来源:J:214959)
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A Lac0-SA-IRES-lacZ-neo/kan cassette was inserted replacing 100 bp of genomic sequence in exon 8 via homologous recombination. Western blot analysis confirmed the absence of protein in brain lysates from P1 homozygous mutant mice. (J:214959)