Rearranged Valpha2-Jalpha27 and Vbeta8.1 Jbeta2.4 fragments were obtained from an HLA-DRB1*0101 (DR1)-restricted A2-specific mouse T cell hybridoma (E168) by RT-PCR. The PCR fragments were subcloned into TCR cassette vectors pTalphacass and pTbetacass and co-injected. (J:223133)