将编码人类KCNIP3(DREAM)的cDNA,其中在EFhand2、3和4位点各插入了两个氨基酸替换(EFmDREAM),插入在人类CaMKII启动子下游。cDNA后面跟着一个IRES和lacZ报告基因。这些氨基酸改变使得突变体对钙离子失去敏感性,从而能阻止DREAM和内源性表达的其他KChIP蛋白的钙离子依赖性去激活功能。(来源:J:103733)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count