在第二外显子上游插入了FRT位点、Splice接受器、EYFP、loxP位点和诺如霉素抗性基因(neo)插入片段,同时在第二外显子下游也插入了一个loxP位点。通过Cre介导的重组,去除了neo基因片段和第二外显子。免疫印迹分析证实了纯合子小鼠中无表达。(来源:J:213772)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
插入,基因内删除
--
1
3
5

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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