Cre介导的重组移除了1到3号外显子,同时插入了耐药性基因 cassette。 Western blot结果显示胸腺细胞中没有蛋白表达。(来源:J:220951)
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Cre-mediated recombination removed exon 1 through 3 and the neomycin resistance cassette inserted. Western blot analysis confirmed the absence of protein expression in thymocytes. (J:220951)