Recombinase mediated cassette exchange (RMCE) on KH2 cells, which contain Gt(ROSA)26Sortm1(rtTA*M2)Jae and an FRT-flanked PGK-Neo-pA in the 3' UTR of the Col1a1 gene, replaced the FRT-flanked neo cassette with a cassette that contains the tetracycline promoter, an EGFP gene and a short hairpin sequence targeting Pten (shPten.1522). (J:212919)