这个定向构建将exon 1的编码序列替换为人类exon 1的编码序列,包含一个loxP位点、一个FRT标记的诺卡因抗性基因座、人类序列从exon 3到13,一个FLAG标签、小鼠3'端非翻译区、一个F3标记的普罗米休斯抗性基因座,再加一个loxP位点和polyadenylation序列。通过Flp介导的重组,两个选择性基因座都被去除了。(来源:J:219255)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
C57BL/6NTac
Targeted
插入,基因内删除
--
1
--
6

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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