Esyt2^tm1Sud

A targeting vector is designed to insert an F3 site followed by a loxP site upstream of exon 10. Inserted downstream of exon 10, from 5' to 3' , are a second loxP site, a FRT site, a neomycin resistance cassette, a second F3 site, a loxP2272 site, and a mutated exon 10 with four aspartate residues mutated into alanine residues (D4A) followed by a second loxP2272 site and a second FRT site. (J:101977)