Lgr4^{tm1.1(cre/ERT2)Npa}

The targeting construct replaced exon 1 following the start codon and 287 bp of intron 1 with an mCherry reporter gene, IRES, inducible cre recombinase fusion (cre/ERT2), polyadenylation sequence and FRT-flanked neomycin resistance cassette. Flp-mediated recombination removed the selection cassette. Antibody testing and direct fluorescence failed to detect mCherry. In situ hybridization confirmed the absence of transcript expression in the intestin, skin and kidney. (J:212415)