一个敲除的诺卡因抗性基因座替换了exon 1a。通过Cre介导的重组,去除了选择性基因座。 Western blot结果显示,大脑中没有检测到a亚型,存在b亚型(来源:J:214695)
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A floxed neomycin resistance cassette replaced exon 1a. Cre-mediated recombination removed the selection cassette. Western blot analysis confirmed the absence of the a isoform and presence of b isoform in the brain. (J:214695)