在肾组织中,通过将lacZ报告基因和诺卡因抗性基因座连接到第5外显子,同时删除了第6外显子, Western blot分析证实了没有蛋白表达(来源:J:209448)
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A lacZ reporter gene followed by a neomycin resistance cassette was fused in frame with exon 5 and deleted exon 6. Western blot analysis confirmed the absence of protein expression in the kidney. (J:209448)