一个敲除的诺卡因抗性基因座替换了17到21号外显子,这些外显子编码部分激酶域。 Western blot分析证实睾丸中没有蛋白表达。(来源:J:213823)
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A floxed neomycin resistance cassette replaced exons 17 through 21, which encodes for part of the kinase domain. Western blot analysis confirmed the absence of protein expression in testes. (J:213823)