通过Cre介导的重组,去除了带有11号外显子的片段。这个等位基因也失去了最初靶向等位基因中的选择性载体。 Western blot结果显示脊髓中没有检测到蛋白(来源:J:213429)
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Cre-mediated recombination removed the floxed exon 11. This allele also lacks the selection cassette found in the originally targeted allele. Western blot analysis confirmed the absence of protein in spinal cord. (J:213429)