外显子2的编码序列被替换为EGFP报告基因和诺卡因抗性元件, Western blot结果显示小鼠胚胎成纤维细胞中没有蛋白表达。(来源:J:219480)
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The exon 2 coding sequence was replaced with an EGFP reporter gene and neomycin resistance cassette. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts. (J:219480)