通过同源重组,将包括exon 1全部的基因区域替换为pgk-neo cassette。 Western blot结果显示,纯合小鼠股骨中没有检测到蛋白表达。(来源:J:210363)
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A genomic region including all of exon 1 was replaced with a pgk-neo cassette via homologous recombination. Western blot analysis confirmed the absence of protein expression in femurs from homozygous mice. (J:210363)