起始密码子,位于exon 2,被替换为AscI酶切位点,以便于AscI-LoxP-EGFPf-3xpA-LoxP-DTR-pA-Frt-PGK-NeoR-Frt-AscI的克隆。通过Flp介导的重组,去除了FRT标记的选择性元件。免疫印迹分析证实了成年纯合突变小鼠中背根神经节(DRG)、脊髓、后肢皮肤和膀胱组织中无蛋白质表达。注意:在突变的DRG和脊髓神经元中,敲入的GFP无法检测到。(来源:J:217933)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129P2/OlaHsd
Targeted
插入
--
1
--
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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