一段来自大鼠cDNA 3'端的3.1kb片段被连接到EGFP后面,然后是一个polyadenylation信号。接着,这段序列与小鼠基因的exon 8中的EcoRI酶切位点融合。同时,还插入了一个条件性荧光素-neomycin cassette,但随后在定向敲除的实验小鼠中被去除了。(来源:J:133999)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count