Igh^{tm3.1(IGH)Vlcg}

Using BAC recombineering roughly 3 Mbp of the mouse locus including all of the Vh Dh and Jh gene segments was replaced with 940 kb of contiguous human sequence containing the equivalent human gene segments. The proximal junction of the replacement was placed about 200 bp downstream from the last J segment and the distal junction was placed several hundred bp upstream of the most 5' V segment of the human gene and about 9 kbp downstream from J558.55. Flp-mediated recombination removed the selection cassette. (J:208619)