Pls1^tm1Fri

A neomycin resistance cassette replaces the second exon of the gene, which contains the start codon. Northern blot analysis indicates expression of low amounts of mRNA of slightly smaller size in intestinal mucosa of homozygotes. Western blot analysis of small intestine lysates indicates absence of protein in homozygotes and further analysis with pan-fimbrin polyclonal antiserum does not find any truncated protein. (J:216723)