Tg(tetO-Nog,-EGFP)53Hda

The transgene has a Pbi-1 bi-directional tetracycline-responsive promoter with an enhanced green fluorescent protein-coding region (eGFP) on one side, and a mouse noggin cDNA sequence (encompassing the complete coding region) on the other side. The promoter contains the Tet-responsive element (TRE) placed between two constitutively active, minimal human cytomegalovirus promoters. The eGFP is followed by a beta-globin polyA signal, and the noggin cDNA is followed by an SV40 polyA signal. Analysis at The Jackson Laboratory revealed two complex transgene integration sites (including one transgene-transgene fusion event) both within the Golgb1 locus on chromosome 16. This two duplications of Golgb1 genome region (totaling ~22.3 kb) are fused to transgene sequences (chr16:36,898,704-36,921,071 [mm10]) and chr16:36,899,753-36,899,756 [mm10]). The number of transgene copies at each site was not determined. The transgene insertion effect on Golgb1 expression has not been specifically determined. (J:216792)