在第12外显子的终止密码子之后,插入了一个IRES-GFP-FRT-PGK-neo-FRT-loxP片段,同时在第10内含子中包含了一个loxP位点。通过Flp介导的重组,移除了PGK-neo片段,剩下11-12号外显子和IRES-GFP被标记为可删除。GFP表达通过流式细胞术和活细胞成像在巨核细胞祖细胞、巨核细胞和LSK细胞中得到了确认。(来源:J:208120)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
插入
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1
7
1

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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