A CAG promoter (cytomegalovirus (CMV) enhancer fused to the chicken beta-actin promoter), FRT-flanked stop cassette, loxP-flanked stop cassette, ReaChR red-shifted channelrhodopsin, fluorescent reporter mCitrine, woodchuck hepatitis post-transcriptional regulatory element (WPRE), poly A (pA) tail, and AttB/AttP-flanked neomycin resistance cassette were introduced to intron 1 of the Gt(ROSA)26Sor gene. PhiC31o-mediated recombination removed the neo cassette. (J:217788)