在exon 1的上游插入了34bp的loxP序列,随后在 intron 1内插入了一个6.4kb的PGK-neo-HsvTK基因座,它被两个loxP位点 flank。通过Cre介导的重组,去除了loxP标记的选择性 cassette和exon 1。逆转录-qPCR证实了杂合雌性的mRNA表达水平降低。(来源:J:208796)
Basic Information
(129X1/SvJ x 129S1/Sv)F1-Kitl+
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count