A ~39 kbp segment was isolated from BAC CTD-2369P2 containing the entire human intercellular adhesion molecule 1 gene (ICAM1), including ~18.9 kbp upstream and ~4.6 kbp downstream. Five founder lines were generated and founder line 4 was determined to have hICAM-1 expression intensity and tissue distribution similar to human. (J:92607)