在目标基因单个外显子的5'端引入了loxP位点,然后在3'端插入了FRT-loxP-(neo-tk)-FRT-loxP(LFNT)元件。通过Cre介导的重组,neo cassette被剔除,留下编码区域被loxP位点包裹。(来源:J:101977, J:211355)
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count