Klk7^em1Rase

Exon 3 was targeted with TALENs. The DNA binding domain of the 5' TALEN targeted GTAAAGAAGGCTCGC, that of the 3' TALEN targeted CTGCTCAAAGGCAATC. Of the four founder lines, founder 'Klk7 ex3-A' was found to have a 20 bp deletion in exon 3 and was selected for further breeding. Western blots confirmed the absence of peptides translated from this allele. The purpose of this allele was to create a double mutant with Klk5. But because the two genes are too close together on the genome for recombination through cross breeding to work efficiently, the TALEN components were directly injected into oocytes carrying the Klk5tm1Rase knock-out allele. (J:238698)