Gcm2^tm1.2Malx

The targeting vector contains a FRT-flanked neomycin selection cassette and a loxP site inserted downstream of exon 2 of the targeted gene, and another loxP site inserted upstream of exon 2. The neomycin cassette was deleted by transient transfection using flp recombinase. Cre-mediated recombination removed exon 2. The deletion creates a frameshift followed by a stop codon that results in the production of a short peptide lacking the main functional domains of the endogenous gene. (J:213334)