Nxf2tm1.1Jw/Y ES细胞通过电穿孔,用pUP108载体构建了Igs8tm1Jw突变,该突变替换小鼠X染色体上Tmsb15a和Armcx5之间一个5.6kb的非基因间隔区,插入了含四条loxP位点的floxed PGK-neo筛选 cassette,形成一个条件性修饰基因。随后,这些含条件性突变的个体与Actb-Cre小鼠杂交,以删除Nxf2和Tmsb15a之间782kb的X染色体区域。(来源:J:210663)
Basic Information
(C57BL/6 x 129S4/SvJae)F1
Legend:
cx: complex: > 1 genome feature ot: other: hemizygous, indeterminate,... (F): Female
(M): Male
N: normal phenotype
(#): related diseases count