在基因组中,一个新霉素抗性基因座替换了包含起始翻译位点的编码外显子。 Western blot 检测证实了在小鼠胚胎成纤维细胞和皮肤中没有蛋白质表达(来源:J:213195)
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A neomycin resistance cassette replaced the coding exon containing the translation initation site. Western blot analysis confirmed the absence of protein expression in mouse embryonic fibroblasts and skin. (J:213195)