设计了一个靶向载体,包含一个单个loxP位点,随后是带有frt内切酶座的诺卡因抗性(neo)基因片段,位于基因的exon 2上游。在exon 2上游,插入了3X Flag序列和另一个loxP位点,位于Nkx2-5的终止密码子之前。通过FLP介导的重组,去除了含frt标记的neo基因片段。(来源:J:101977)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
129X1/SvJ
Targeted
插入
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1
23
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Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

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PMID
Journal
Year
IF
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