Shcbp1^{tm1d(EUCOMM)Wtsi}

The L1L2_Bact_P cassette was inserted at position 4798418 of Chromosome 8 upstream of the critical exon(s) (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon(s) at position 4800268. The critical exons are thus flanked by loxP sites. The lacZ and neomycin segments were excised with germline Flp recombinase, leaving exons 4 and 6 flanked by loxP sites. Then animals were crossed with a germline Cre strain to delete exons 4 through 6. Further information on targeting strategies used for this and other IKMC alleles can be found at http://www.informatics.jax.org/mgihome/nomen/IKMC_schematics.shtml. (J:212898)