Prg4^tm2Mawa

A targeting vector containing a loxP-flanked artifical exon that contains a strong splice acceptor, an in-frame Bgeo lacZ coding sequence, an internal ribosome entry site (IRES), a reverse tet transactivator (rtTA) cassette with a polyA signal, and a neomycin selection cassette driven by its own promoter was inserted into intron 2 of the gene. The allele does not express wild-type Prg4 mRNA, as determined by RT-PCR. Cre-mediated excision of the targeting vector leaves one loxP in intron 2 and allows expression of Prg4 mRNA. (J:101977)