Mgat4b^tm1.1Jxm

A targeting vector was used to place a floxed neomycin-thymidine kinase expression cassette downstream of exons 2, 3, and 4, and a loxP site upstream of the exons. Cre-mediated recombination removed the neo cassette and exons 2-4 leaving a residual loxP site. Removal of exons 2-4 causes a frameshift at the junction of exon 1 and 5, generating a new translational termination codon that results in a truncated protein lacking the catalytic domain. (J:212313)