Fzd6^tm2.1Nat

LoxP sites were inserted into introns 3 and 4 in order to flank exon 4 (the largest coding exon in the gene). A FRT-neo-FRT cassette that was used for positive selection was introduced adjacent to the loxP site in intron 3. FLP-mediated recombination removed the FRT-flanked neo cassette. (J:228792)