Gt(ROSA)26Sor^{tm8(cre/Esr1*)Nat}

The targeting vector contains (from 5' to 3') a splice acceptor, IRES, Mer-Cre-Mer, a bovine growth hormone 3' UTR, and a Frt-PGKneo-Frt cassette. The MerCreMer double fusion protein has a Cre recombinase cDNA sequence flanked on each end with a mutated murine estrogen receptor (mer) ligand binding domain (amino acids 281-599, G525R); thus rendering Cre expression tamoxifen-inducible yet estrogen-insensitive. (J:101977)