这个靶向构建在exon 2上游插入了FRT位点、Splice接受器、IRES、β-geo盒、poly(A)序列、另一个FRT位点和loxP。在exon 2下游,额外插入了一个loxP位点。通过Flp介导的重组,去除了含FRT标记的β-geo盒。随后,Cre介导的重组移除了exon 2。反转录PCR证实了小肠中转录表达的缺失。(来源:J:211898)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
Not Specified
Targeted
插入,基因内删除
--
1
--
2

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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