BAC bMQ317G18 was engineered to contain the insertion of the mKate2 (a far-red fluorescent protein), IRES, puromycin resistance cassette, polyadenylation sequence, loxP site, and F3-flanked neomycin resistance cassette into Nanog at the translation start site in exon 1. The pound symbol (#) is used when no line is specified and/or lines are pooled. (J:200557)