在Slc1a2基因的第2内含子插入了loxP位点,然后在第4内含子插入了frt-neo-frt-loxP序列,通过这个设计,细菌人工染色体克隆RP24-315H4中,3号和4号外显子被FRT-loxP系统标记为可删除。通过FLP介导的重组,Neomycin cassette被去除了,留下3号和4号外显子由loxP位点控制的内含子。接下来,通过Cre介导的重组,可以消除3号和4号外显子,导致产生一个框移的缺失突变。(来源:J:207177)

Basic Information

Allele
Strain of Origin
Allele Type
Mutation
Inheritance
Related Gene
Related Disease
Reference
(129S6/SvEvTac x C57BL/6J)F1
Targeted
插入
--
1
2
5

Phenotypes

Legend:
hm: homozygous
ht: heterozygous
cn: conditional genotype
cx: complex: > 1 genome feature
tg: involves transgenes
ot: other: hemizygous, indeterminate,...
(F): Female
(M): Male
phenotype observed
N: normal phenotype
(#): related diseases count
Phenotypes:
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Phenotypes

References Literature

Title
PMID
Journal
Year
IF
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