Gt(ROSA)26Sor^{tm1(MCPyV168)Jdec}

The Merkel cell polyomavirus (MCPyV) early region, isolated from MCC tumor specimen MCCw168 (MCPyV168; GenBank: KC426954.1), was PCR amplified and cloned into the pBigT vector between XhoI and NotI restriction sites. A 6kb fragment containing the neomycin resistance gene and triple SV40 polyadenylation transcription terminators flanked by two loxP sites (loxP-stop-loxP, or LSL), MCPyV168, and bovine growth hormone polyadenylation terminator were cut from the pBigT vector and cloned into the pROSA26PA vector between PacI and AscI restriction sites. (J:219802)