Tg(Cck-cre,-lacZ)1Mini

A BAC (RP23-26B6) containing the Cck gene was modified by ET recombination so that a cassette composed of a hybrid intron, a cre recombinase gene, an internal ribosomal entry site (IRES), a beta-galactosidase gene (lacZ), and a neomycin/kanamycin resistance marker was inserted into the first ATG of the first coding exon (exon 2). The neo/kan marker was removed using flp recombinase. The purified construct was injected into the pronuclei of (CBA x C57BL/6) hybrid oocytes. (J:210229)