Piezo2^tm2.1Apat

A targeting vector was constructed to insert loxP sites upstream of exon 43 and downstream of exon 45 of the Piezo2 gene. The vector contained an frt-flanked PGK-neo cassette upstream of the first loxP site. Following homologous recombination in ES cells, the PGK-neo marker and a single loxP site were upstream of exon 43 and a second loxP site was downstream of exon 45.Chimeric animals were crossed to EIIa-cre expressing mice to delete exons 43-45, causing a frameshift that results in production of a premature stop codon. (J:211312)